ABSTRACT
Objective To establish male rat models for fertility following liver transplantation. Methods Male Sprague-Dawley (SD) rats were used as the donors and recipients of liver transplantation. The donor liver was transplanted with two-cuff technique. Liver transplantation was performed in 15 male SD rats. At 3 weeks after liver transplantation, 5 rats were randomly sacrificed for detection of sperm deformity rate. The remaining male rats were mixed bred and mated with healthy female SD rats at a ratio of 1︰2. General conditions of the rats undergoing liver transplantation were recorded. Liver function parameters were detected after liver transplantation. Postoperative sperm deformity rate was observed. The pregnant status of female rats and health situation of their offsprings was monitored. Results All 15 rats (100%) underwent liver transplantation successfully. Nine rats (9/10) survived longer than 8 weeks. Liver function parameters were normal in male rats following liver transplantation. The sperm deformity rate was ranged from 0.5% to 1.3%. Ten male rats undergoing liver transplantation were mixed bred with female rats at a ratio of 1︰2 for 1 week. All female rats were successfully mated and delivered their offsprings after 3 weeks. The offsprings had no evident physiological deformity. Conclusions Male rat models for fertility are successfully established after liver transplantation, which serve as an animal model to evaluate the fertility performance in male patients undergoing liver transplantation.
ABSTRACT
Objective To analyze the fertility rate and to estimate the future population size of Shaanxi province,based on data from the sixth national population census.Methods Fertility rate curve was used to analyze the fertility model and the abbreviated life table.The actual fertility rate was used as the main way to predict the future population size.General fertility rate was analyzed by factor analysis approach.Results The total fertility rate of Shaanxi province was 1.05 in 2010 while age-specific fertility rate contributed 101.27％ to the general fertility rate.The expected population sizes would be 38 122 474 in 2015,38 432 931 in 2020 and 38 121 904 in 2025 respectively.Conclusion Birthrate would become lower and the population size appearing a negative increase in the year 2020,in Shaanxi province.